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Pack Size | Price | Availability | Quantity |
---|---|---|---|
10 mg | $39 | In Stock | |
25 mg | $59 | In Stock | |
50 mg | $79 | In Stock | |
100 mg | $132 | In Stock |
Description | Propidium Iodide (PI) is a red fluorescent dye that can be used for cell staining and is suitable for fluorescence microscopy, confocal microscopy, flow cytometry, and fluorometer analysis. In aqueous solution, the Ex/Em of Propidium Iodide is 493/636 nm. And after binding with nucleic acid, the Ex/Em of PI-nucleic acid becomes 535/617 nm, while the fluorescence signal is enhanced 20-30 times. |
In vitro | METHODS: Propidium Iodide uptake and Flow cytometry to detect cell death: 1. Store the PI stock solution (0.5 mg/mL in PBS) in a dark place at 4℃. Immediately before use, prepare PI-FACS buffer by adding 20 µL of PI stock solution per 1 mL of PBS. 2. Collect suspended cells: Collect cells directly in centrifuge tubes and centrifuge at 500g for 5 min to harvest all cells. 3. Collect adherent cells: Remove and preserve the medium containing dead and mitotic cells. Isolate live cells using standard tissue culture techniques, such as incubation with trypsin-EDTA, and be sure to collect any washings (e.g., PBS). Add cells from the culture medium and cells from any wash solution to the isolated cells and centrifuge at 500 g for 5 min to harvest all cells. 4. Resuspend the harvested cells in PI-FACS buffer. The cells were incubated in the dark for 15 min at room temperature. 5. Determine the cell mortality rate by flow cytometry. [1] |
Alias | PI |
Molecular Weight | 668.39 |
Formula | C27H34I2N4 |
Cas No. | 25535-16-4 |
Storage | keep away from direct sunlight Powder: -20°C for 3 years | In solvent: -80°C for 1 year | ||||||||||||||||||||||||||||||||||||||||
Solubility Information | H2O: 5 mg/mL (7.48 mM) DMSO: 6.68 mg/mL (10 mM) | ||||||||||||||||||||||||||||||||||||||||
Solution Preparation Table | |||||||||||||||||||||||||||||||||||||||||
DMSO/H2O
DMSO
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